Journal: Nucleic Acids Research

Article Title: RalR (a DNase) and RalA (a small RNA) form a type I toxin–antitoxin system in Escherichia coli

doi: 10.1093/nar/gku279

Figure Lengend Snippet: RalR cleaves DNA. ( A ) RalR cleaves lambda DNA after 30 min and 120 min. ( B ) RalR cleaves unmethylated lambda DNA ( dam − , dcm − ) after 30 min and 120 min. In (A) and (B), the positive control is degradation of DNA by DNase I, and the negative control is the inactive RalR-mutant (RalR K52Q). EDTA blocks RalR and DNase I activity. ( C ) RalR activity requires co-factor Mg 2+ and/or Ca 2+ .

Article Snippet: The equal amount of deoxyribonuclease I (DNase I; New England Biolabs, Beverly, MA, USA) was used as a positive control, and excess EDTA (20 mM) was added in the reaction to inhibit the nuclease activity.

Techniques: Lambda DNA Preparation, Positive Control, Negative Control, Mutagenesis, Activity Assay

Journal: Tissue antigens

Article Title: Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules

doi: 10.1111/tan.12095

Figure Lengend Snippet: (A) HLA class I allotypes represented by the One Lambda Labscreen and Gen-Probe LifeCodes beadsets. (B) Binding of the monomorphic HLA class I antibody W6/32 to beads from One Lambda LabScreen (grey bars) and Gen-Probe LifeCodes (orange bars). The allotypes shown are those common to both beadsets.

Article Snippet: The specific reference as listed in the current publication is noted to the right. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 (A) HLA class I allotypes represented by the One Lambda Labscreen and Gen-Probe LifeCodes beadsets. (B) Binding of the monomorphic HLA class I antibody W6/32 to beads from One Lambda LabScreen (grey bars) and Gen-Probe LifeCodes (orange bars).

Techniques: Binding Assay

Journal: Tissue antigens

Article Title: Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules

doi: 10.1111/tan.12095

Figure Lengend Snippet: (A) Binding of MA2.1 (1μg/ml) to beads coated with HLA class I allotypes from the One Lambda LabScreen (left panel) and Gen-Probe LifeCodes (right panel) beadsets. (B) Alignment of HLA class I allotypes showing selected residues in the α1 and α2 domains. Residues from allotypes that form the epitope recognized by MA2.1 are shaded in grey. (C) Space-filling model of the binding surface of HLA-A*02 (grey) with associated peptide (cyan). Residues highlighted in yellow fall within the footprint recognized by MA2.1. Residues 62–65 are critical for formation of the epitope recognized by MA2.1 and are highlighted in red.

Article Snippet: The specific reference as listed in the current publication is noted to the right. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 (A) HLA class I allotypes represented by the One Lambda Labscreen and Gen-Probe LifeCodes beadsets. (B) Binding of the monomorphic HLA class I antibody W6/32 to beads from One Lambda LabScreen (grey bars) and Gen-Probe LifeCodes (orange bars).

Techniques: Binding Assay

Journal: Tissue antigens

Article Title: Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules

doi: 10.1111/tan.12095

Figure Lengend Snippet: (A) Binding of PA2.1 (1μg/ml) and BB7.2 (1μg/ml) to beads coated with HLA class I allotypes from the One Lambda LabScreen (left panel) and Gen-Probe LifeCodes (right panel) beadsets. (B) Binding of PA2.1 (50μg/ml) and BB7.2 (50μg/ml) to beads coated with HLA class I allotypes from the One Lambda LabScreen (left panel) and Gen-Probe LifeCodes (right panel) beadsets. (C) Alignment of HLA class I allotypes showing selected residues in the α2 domain. Residues from allotypes that form the epitope recognized by PA2.1 and BB7.2 are shaded in grey. (D) Space-filling model of HLA-A*02 (grey) with associated peptide (cyan). Residues highlighted in yellow fall within the footprint recognized by PA2.1 and BB7.2. Tryptophan at position 107 is considered critical for formation of the epitope recognized by PA2.1 and BB7.2 and is highlighted in red.

Article Snippet: The specific reference as listed in the current publication is noted to the right. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 (A) HLA class I allotypes represented by the One Lambda Labscreen and Gen-Probe LifeCodes beadsets. (B) Binding of the monomorphic HLA class I antibody W6/32 to beads from One Lambda LabScreen (grey bars) and Gen-Probe LifeCodes (orange bars).

Techniques: Binding Assay

Journal: Tissue antigens

Article Title: Direct binding to antigen-coated beads refines the specificity and cross-reactivity of four monoclonal antibodies that recognize polymorphic epitopes of HLA class I molecules

doi: 10.1111/tan.12095

Figure Lengend Snippet: (A) Binding of BB7.1 (1μg/ml) to beads coated with HLA class I allotypes from the One Lambda LabScreen (left panel) and Gen-Probe LifeCodes (right panel) beadsets. (B) Alignment of HLA class I allotypes showing selected residues in the α1 and α2 domains. Residues from allotypes that form the epitope recognized by BB7.1 are shaded in grey. (C) Space-filling model of the binding surface of HLA-B*07 (grey) with associated peptide (cyan). Residues highlighted in yellow fall within the footprint recognized by BB7.1. Residues 63–71 in the a1 domain and position 131 in the α2 domain are critical for formation of the epitope recognized by BB7.1 and are highlighted in red.

Article Snippet: The specific reference as listed in the current publication is noted to the right. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 2 caption a7 (A) HLA class I allotypes represented by the One Lambda Labscreen and Gen-Probe LifeCodes beadsets. (B) Binding of the monomorphic HLA class I antibody W6/32 to beads from One Lambda LabScreen (grey bars) and Gen-Probe LifeCodes (orange bars).

Techniques: Binding Assay

Journal: STAR Protocols

Article Title: DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

doi: 10.1016/j.xpro.2023.102247

Figure Lengend Snippet:

Article Snippet: Klenow DNA polymerase (3′ – 5 ′ exo-, 50 U/μL) , TIANGEN , NG202-02.

Techniques: Recombinant, Lambda DNA Preparation, Methylation, Multiplex Assay, Software, Stripping Membranes, Microinjection

Journal: STAR Protocols

Article Title: DNA methylation protocol for analyzing cell-free DNA in the spent culture medium of human preimplantation embryos

doi: 10.1016/j.xpro.2023.102247

Figure Lengend Snippet:

Article Snippet: Klenow DNA polymerase (3′ – 5 ′ exo-, 50 U/μL) , TIANGEN , NG202-02.

Techniques: Concentration Assay

Journal: FEMS microbiology letters

Article Title: Genome size and macrorestriction map of Xanthomonas campestris pv. glycines YR32 chromosome.

doi: 10.1111/j.1574-6968.1999.tb13602.x

Figure Lengend Snippet: Fig. 1. Pulsed-¢eld gel electrophoresis (PFGE) of X. campestris pv. glycines YR32 genomic DNA digested with PacI, SwaI, and PmeI (50^650-s pulse, 50-h run). A: PacI, SwaI and PacI-SwaI digestion. Lanes: 1, yeast chromosomal standards; 2, X. campestris pv. glycines YR32 genomic DNA digested with PacI; 3, PacI-SwaI digest; 4, SwaI digest; 5, R. sphaeroides 2.4.1 genomic DNA digested with SpeI as molecular size markers. B: PmeI, SwaI and PmeI-SwaI digestion. Lanes: 1, yeast chromosomal standards; 2, PmeI digest; 3, PmeI-SwaI digest; 4, SwaI digest; 5, SpeI-digested R. sphaeroides 2.4.1 markers. C: PacI, PmeI and Pac-PmeI digestion. Lanes: 1, yeast chromoso- mal standards; 2, PacI digest; 3, PacI-PmeI digest; 4, PmeI digest; 5, SpeI-digested R. sphaeroides 2.4.1 markers.

Article Snippet: For AseI and SpeI digests, lambda concatemeric DNA (New England Biolabs) and AseI-digested R. sphaeroides 2.4.1 genomic DNA [26] were used as a molecular size standards.

Techniques: Nucleic Acid Electrophoresis

Journal: FEMS microbiology letters

Article Title: Genome size and macrorestriction map of Xanthomonas campestris pv. glycines YR32 chromosome.

doi: 10.1111/j.1574-6968.1999.tb13602.x

Figure Lengend Snippet: Fig. 2. PFGE of X. campestris pv. glycines YR32 genomic DNA digested with AseI and SpeI. A: Optimal separation of the larg- est DNA fragments (25^70-s pulse, 24-h run, 180 V). Lanes: 1, concatemeric lambda DNA; 2, R. sphaeroides 2.4.1 genomic DNA digested with AseI as molecular size markers; 3, X. cam- pestris pv. glycines YR32 genomic DNA digested with AseI; 4, SpeI digest; 5, yeast chromosomal standards. B: Optimal separa- tion of small- and medium-sized DNA fragments (20^25-s pulse, 22-h run, 180 V). Lanes: 1, AseI-digested R. sphaeroides 2.4.1 markers; 2, X. campestris pv. glycines YR32 digested with SpeI; 3, AseI digest.

Article Snippet: For AseI and SpeI digests, lambda concatemeric DNA (New England Biolabs) and AseI-digested R. sphaeroides 2.4.1 genomic DNA [26] were used as a molecular size standards.

Techniques: Lambda DNA Preparation

Journal: FEMS microbiology letters

Article Title: Genome size and macrorestriction map of Xanthomonas campestris pv. glycines YR32 chromosome.

doi: 10.1111/j.1574-6968.1999.tb13602.x

Figure Lengend Snippet: Fig. 4. Southern hybridization using the pat gene as a probe. A: PFGE of X. campestris pv. glycines YR32 genomic DNA digested with PacI, PmeI, SwaI and multiple digests. Lanes: 1, R. sphaeroides 2.4.1 genomic DNA digested with SpeI as molecular size markers; 2, X. campestris pv. glycines YR32 genomic DNA digested with PacI; 3, PmeI digest; 4, SwaI digest; 5, PacI-PmeI digest; 6, PmeI-SwaI di- gest; 7, PacI-SwaI digest; 8, yeast chromosomal standards. B: Southern hybridization result.

Article Snippet: For AseI and SpeI digests, lambda concatemeric DNA (New England Biolabs) and AseI-digested R. sphaeroides 2.4.1 genomic DNA [26] were used as a molecular size standards.

Techniques: Hybridization

Journal: FEMS microbiology letters

Article Title: Genome size and macrorestriction map of Xanthomonas campestris pv. glycines YR32 chromosome.

doi: 10.1111/j.1574-6968.1999.tb13602.x

Figure Lengend Snippet: Fig. 5. PFGE of X. campestris pv. glycines YR32 digested with I-CeuI and subsequent digestion with PacI, PmeI, and SwaI. Lanes: 1, R. sphaeroides 2.4.1 genomic DNA digested with SpeI as molecular size markers; 2, I-CeuI digest; 3, I-CeuI-PacI di- gest; 4, I-CeuI-PmeI digest; 5, I-CeuI-SwaI digest; 6, I-CeuI- PacI-PmeI digest; 7, I-CeuI-PmeI-SwaI digest; 8, I-CeuI-PacI- SwaI digest; 9, yeast chromosomal standards.

Article Snippet: For AseI and SpeI digests, lambda concatemeric DNA (New England Biolabs) and AseI-digested R. sphaeroides 2.4.1 genomic DNA [26] were used as a molecular size standards.

Techniques: